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The advantage but also challenge of a multiplex array is the combination of several assays on a single platform.
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Within the anti-CCP-2-negative subset, positivity in the multiplex array was not associated with progression (30%% in multiplex-negative versus 30%% in multiplex-positive).
The reproducibility of the bead-based multiplex array was determined by establishing inter-assay and intra-assay coefficients of variance (CVs).
To search for other potentially important cell recruitment mediators, a multiplex array was performed on a subset of end-stage lung disease patients' plasma.
Within anti-CCP-2-negative patients, ACPA reactivity as detected by multiplex array was not significantly associated with known risk factors or clinical or prognostic parameters.
Through combinatorial analysis of all 3 biomarkers using optimal cutoff values defined by Youden index calculations, the AUROC for the diagnostic panel using the multiplex array was 0.9476 [95% CI: 0.903 - 0.992].
A major risk in the multiplex arrays is the increased cross-reactivity, due to the presence of multiple antibodies, which are normally applied as a mixture.
Multiplex bead array is compared to the "gold standard," ELISA.
Recently, a multiplex protein array was also employed, providing high-throughput analysis [ 61]; however, this method requires some prior knowledge of likely analytes.
The array is a multiplex fluorescent sandwich ELISA that contains internal normalization, standards for quantification, and is optimized for cell culture supernatants.
In addition, for Agilent several multiplex array formats are available.
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