We have recently, with the help of our engineering collaborators, streamlined and multiplexed RNA aptamer selections.
Rapid identification of cell-specific, internalizing RNA aptamers with bioinformatics analyses of a cell-based aptamer selection.
The method for nucleic acid aptamer selection is quite simple and yields highly specific aptamers within a short time [ 4, 6].
Although SELEX has proven to generate specific aptamers for several targets, MonoLEX can be a valuable alternative for aptamer selection.
To select DNA aptamers specific for Vaccinia virus (VACV) as a model for OPV, aptamer selection was performed in two steps.
We anticipate that the cell-based aptamer selection approach used here will be broadly applicable to the identification of aptamer-based agonists for a variety of cell-surface signaling receptors.
Yüce M. Trends in aptamer selection methods and applications.
Yuce, M., Ullah, N. & Budak, H. Trends in aptamer selection methods and applications.
We were the first to demonstrate aptamer selection to distinct surfaces of a target, which is important for teasing apart different functions of a factor, and first to demonstrate aptamer inhibition of a protein's function in an animal.
Here, we summarize key recent developments in aptamer selection methods, as well as other aspects of aptamer selection that have significant impact on the outcome.
