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Until now no multiplex approach is available for the subtyping of IV antibodies based on HA and NA serotype differentiation.
Another challenge is when a multiplex approach is required, where multiple independent quantities are measured for each single cell.
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It was shown that the assay allowed accurate detection of the MLL fusion gene, regardless of the breakpoint location, and confirmed that this multiplex approach was robust in a global multicenter trial.
A multiplex approach was essential since patient responses were heterogeneous.
To reduce the complexity and cost of the NGS analysis, a multiplex approach was tested, whereby eight samples were examined in parallel (8-plex).
The multiplex approach was implemented in this pulse sequence by changing the phase cycles of the excitation pulses prior to the evolution times to be incremented in three steps of 120°.
In a recent study by Chandra et al. [ 26], another multiplex approach was described for the detection of ACPAs, by using a chip design with up to 10 antigens per chip.
Therefore, a parallel multiplexing approach is gaining the interest as an alternative to overcome the limitations of multicolor-based IHC and to enhance throughput of biomarker multiplexing.
Therefore, multiplex approaches are used to understand the customer behavior integrating multiple data sources and multiple types of data [7] to reach the combined prediction results.
Multiplex approaches are likely to be most effective and, with appropriate translational support, could be practicable.
To this end, application of our multiplex HTM approach is underway with patient-derived genital skin fibroblasts (Szafran, Mancini and Marcelli, unpublished observations).
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