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Since multiplex analysis is a relatively new methodology and a number of studies have validated the accuracy of the approach, some discrepancies have also been observed between multiplex assays and ELISA [ 26- 30].
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Multiplex analysis was performed on Luminex 100 IS System (Luminex, Austin, TX, USA) and with Luminex 100 IS 2.3 and HLA Fusion 2.0 software.
A multiplex analysis was performed to evaluate levels of 17 cytokines.
Consequently, multiplex analysis was used as the primary method of measuring pSTAT for all further studies reported here [ 25].
Plasma was separated by centrifugation at 1000 rpm for 5 min and stored at -20 until the multiplex analysis was performed.
Multiplex analysis was undertaken on synovial fluid samples from normal, early OA, OA and RA joints to investigate protein expression levels of MMPs and TIMPs.
Luminex cytokine multiplex analysis was performed in order to evaluate variations in the expression of cytokines and chemokines in our serum and tissue samples.
Multiplex analysis was performed on the culture supernatants of this in-house assay to identify test read-outs alternative to interferon-gamma that could increase the sensitivity of the test.
Since the number of fetal DNA molecules in maternal plasma is limited, identification of multiple markers to perform multiplex PCR analysis is suggested to increase the number of molecules that can be counted and hence improving the precision for the EGG ratio estimation.
Multiplex cytokine analysis was performed on conditioned media collected from primary microglia and evaluated using Millipore bead array and analyzed via Luminex 100IS system (n = 3 separate experiments).
Tail clips were lysed and multiplex PCR analysis was used to genotype the mice using RED Extract-N-Amp Extract-N-Amp Extract-N-Amp PCRrs: F (5'-GGATAGGATGGCTACTAGCTGC), R1 (5'-TTACTAGCTAAGTGGCCCAGG) and T (see above).
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