Based on the results of the genetic distance and clustering analysis, the accessions classified as japonica rice were used to evaluate the performance of the three marker panels in comparison to previously reported multiplex marker analyses using the program PowerMarker v.3.23 [ 45].
Multiplex analyses were used to quantify levels of C-reactive protein (CRP), Fractalkine, Interleukin-1 receptor-antagonist (IL-1RA), interferon-alpha (IFNalpha), interferon-gamma (IFNgamma), Interferon gamma-induced protein 10 (IP-10), Macrophage inflammatory protein-1beta (MIP-1beta), and Vascular Endothelial Growth Factor (VEGF) in 24-h-urine.
This can be achieved either by integrating the analyses using a single platform, such as the multiplex immunoassay method described above, or by using sophisticated bioinformatic and biostatistical methods to integrate the metabolite and proteomic data acquired using different platforms.
Multiplex data was analysed using a three-way repeated measures ANOVA with mixed models or a generalized estimating equations (GEEs) model, depending on the distribution of data.
Microsatellite markers BAT-25, BAT-26, NR-21, NR-22, and NR-27 were amplified in multiplex reaction and analysed using DHPLC and capillary electrophoresis (CE).
The primers, protocols, and analyses used for multiplex PCR were performed as previously described [ 26, 27].
GHR variants, wild-type (fl) and deleted for exon 3 (d3) were analysed using multiplex PCR.
The aim of this study was to analyse, using multiplex technology, the autoantibodies predating the onset of symptoms of SLE in individuals in a patient population in northern Europe and to relate these autoantibodies to the first recorded symptom of disease.
Their DNA was analysed using a Multiplex Real-Time PCR Assay (Islam et al. 2015) at the USDA-APHIS Center for PLaboratoryh Science and Technology, Otis LaBuzzards Bayzzards Bay, MA, USA.
Plasma samples were analysed using a multiplex ELISA.
