Sentence examples for multiplex amplification is from inspiring English sources

Whilst LAMP is inexpensive to perform, primer design is complex and multiplex amplification is at present difficult.

For this subset of markers the possibility of multiplex amplification was tested, as were the variability and allele range of these markers.

Multiplex amplification was performed in two different ways.

However, the capacity of multiplex amplification was limited by interaction between primers.

This screening detected 16 polymorphic loci that consistently amplified (Table 1); however, only 12 with adequate sizes for multiplex amplification were selected to genotype the whole set of samples.

Multiplex amplification was successful under such conditions, and we finally tested DNA of 44 samples from two natural populations of A. miyabei subsp. miyabei f. miyabei at Bibi and Kyouwa in Chitose city, Hokkaido, Japan (Appendix 1).

Multiplex amplifications were performed in mixtures containing 1.5 mM MgCl2, 0.2 mM dNTPs and 0.05 U of Taq recombinant polymerase per µl.

Multiplex amplifications were carried out in a total volume of 15 μL.

The first multiplex amplifications were carried out using equal concentration of locus specific forward and reverse primers (0.2 μM each) to screen eight individuals.

An additional important problem to address for multiplex PCR amplification is the potential lack of specificity.

This method, called multiplex ligation-dependent probe amplification, is a quantitative multiplex PCR approach to determine the relative copy number of each MLH1 and MSH2 exon.