As we found no effect of CR on rDNA silencing, we investigated whether deletion mutations that reproducibly extend lifespan in multiple yeast background strains alter rDNA silencing.
Asterisks indicate significant difference (p values are based on paired t tests corrected for multiple comparisons) between the yeast strains harboring cocoa genes and YJ0.
A mixture of multiple substrate-selective microbial or yeast strains is expected to act in concert to simultaneously uptake pentose and hexose sugars and efficiently convert to value-added bioproducts (Suriyachai et al. 2013; Henson and Hanly 2014).
Our results show that natural yeast strains harbor multiple specific alleles of genes controlling essential functions, that are apparently compatible with survival in the natural environment.
These newly identified alleles can be used as gene tools for engineering industrial yeast strains with multiple subtle changes, minimizing the risk of negatively affecting other essential functions.
The aim of our current study was to develop a new technology to create yeast strains carrying multiple exogenous genes by integrating them into precisely engineered loci in the yeast genome.
A modified TAP (10× histidine-TEV-ProteinA) tag mTAP was fused to the C terminus of the Med14, Med17, Med22 or Med21 subunit, using a PCR-based genomic epitope-tagging method in the multiple protease-deficient yeast strain BJ2168 (MATa leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 galeu2
Yeast hosts have been demonstrated to be capable of expressing multiple free cellulases within single-cell yeast strains, such as Saccharomyces cerevisiae [ 95], Saccharomyces pastorianus [ 96], Pichia pastoris [ 97, 98] and more recently the Kluyveromyces marxianus [ 99].
The main objectives of this study were to screen for yeast strains displaying tolerance to multiple stresses present during high-temperature ethanol fermentation, i.e., heat, ethanol, osmotic, oxidative, and cell wall stresses, and to investigate cellular mechanisms responsible for multiple stress tolerance in these strains.
There are multiple strategies developed that aim to provide suitable yeast strains for specific industrial goals.
