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Loss of the mwh+Y chromosome in any of the wing-disk cells – in a multiple wing hairs homozygous background – leads to the formation of an mwh mosaic spot (clone) in the emerging wing.
Multiple wing hairs can also be generated by overexpressing core PCP proteins such as Fz.
Furthermore, a high occurrence of multiple wing hairs was observed (Fig. 1E).
Interestingly, a dominant enhancement was also observed with fy and mwh, two genes whose mutant phenotype are multiple wing hairs.
Flies heterozygous for a mutation in the multiple wing hairs (mwh) gene were used for all experiments on LOH [33], [34].
The removal of one copy of dGIPC did however not modify the GOF phenotype of Fz, Stbm, Pk, Dgo or Fmi, both in terms of the specific swirling patterns observed and in the number of multiple wing hairs formed.
Similar(45)
The dGIPC gain of function (GOF) phenotype was a strong multiple wing hair defect (see above).
Stabilization of an actin pool is consistent with the multiple wing hair phenotype seen in the adult.
Mutations in the genes more directly related to wing hair formation typically give a multiple wing hair phenotype and/or deformed and split wing hairs.
Strikingly, an enhancement of the multiple wing hair phenotype of overexpressed dGIPC was observed when removing one copy of the small GTPase cdc42.
In order to identify the genes affected downstream of overexpressed dGIPC, we have assayed for modification of the strong multiple wing hair phenotype associated with GS2053 by removing one copy of candidate genes.
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