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We thank Dr Chunji Liu for providing seed for screening multiple wheat genotypes.
We conducted a genome-wide survey of wheat miRNAs from multiple wheat tissues of different developmental stages.
The gene was supported by multiple wheat full-length ESTs (CJ799192, BE500656, BG263254, BQ170023) all with a greater than 95% nucleotide identity.
In this study, in order to discover wheat miRNAs in whole genome scale by experimental approach, we identified 689 miRNAs from multiple wheat tissues of different developmental stages based on all of the genome sequences available.
The R5-ELISA responds to a peptide sequence that is present in gliadin and then reports the amount of gluten by referencing to a gliadin material based on multiple wheat cultivars (Van Eckert et al. 2006).
The development of a non-denaturing polyacrylamide detection system makes TILLING more accessible to a larger set of researchers and breeding programs and may facilitate the development of multiple wheat TILLING populations.
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However, we also found multiple wheat-specific variants of conserved miRNAs exhibiting nucleotide substitutions as compared to other species.
It has been reported that multiple pretreatments of wheat straw consisting of BM followed by various chemical treatments were not promising compared with single chemical pretreatments [ 32].
In bread wheat, multiple protein alleles were discovered for each of the three Glu-3 loci based on their differences in electrophoretic mobility in SDS-PAGE [52], and these alleles differed in their effects on certain aspects of bread-making quality [53] [55].
They were distributed across two to three bins on each chromosome, indicating that the contig spans multiple bins on wheat group 1 chromosomes.
The generation of multiple TILLING libraries in wheat will allow an even greater flexibility and robustness to wheat TILLING as any targets which are missing in one population could be screened for in others.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com