Variables used in the model were age, gender, symptom score and multiple virus detection.
There were no associations between multiple virus detection with age or severity of pneumonia (data not shown).
New assays using multiplex reverse transcriptase polymerase chain reactions (MRT-PCR) are available allowing for relatively rapid detection of multiple virus types and simultaneous comparison of patient characteristics, symptoms, severity of illness and productivity across multiple viruses.
Recently, a new molecular biology-based microbial detection method for rapid identification of multiple virus types in one specimen has been developed.
To the Editor: Since the first detection of the influenza A(H5N1) virus in geese in China during 1996 (http://www.cdc.gov/flu/avianflu/h5n1-virus.htm), H5 subtype viruses have continued to reassort and evolve, giving rise to multiple virus clades and gene constellations.
Data on influenza virus detections was obtained from three sources.
Influenza viruses B (n = 7,150; 40% of all influenza virus detections) became dominant in week 8.
#Includes 211 enterovirus, 3 coxsackie B3 virus, and 1 coxsackie B5 virus detections.
Pandemic influenza A (H1N1 2009) detections peaked in 2010 (41.7% of all influenza virus detections).
The severity of clinical signs and symptoms, including diarrhea, induced by infection with H5N1 indicated a rapid spread of the virus4 confirmed by virus detection in multiple organs, including the brain (Table 2), using the real-time PCR technique.
