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Patients (N = 192) attending an emergency department during the 2007 2008 respiratory season consented, and "excess" frozen archived nasopharyngeal aspirates were analysed; 46 were positive by conventional virology and 69 by RT-PCR/ESI-MS, among which there were six samples with multiple viral pathogens detected.
One or multiple viral pathogens were detected in respiratory specimens of 222 of 309 (72%) patients.
Therefore, the significance of the detection of multiple viral pathogens in ARIs is unclear.
The application to discriminate among variants of FMDV is added to a number of microarray procedures used in virology to analyze multiple viral pathogens that belong to different virus families [ 18, 33], to detect specific viruses [ 34- 36] or to define genetic variations underwent by viruses [ 37, 38] [reviews in [ 39, 40]].
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BRDC is a multi-factorial disease with environmental conditions interacting with multiple viral and bacterial pathogens to produce severe respiratory illness.
If extreme weather events become increasingly frequent owing to global climate change, the consequent synchronization of proliferating pathogens or their vectors may cause disease to become a major threat to historically stable populations that had previously coexisted with multiple viral and parasitic pathogens.
Previous studies have also identified CD46 as a host receptor for multiple viral and bacterial pathogens, such as measles virus, herpes virus 6, adenovirus and S. pyogenes.
Multiple (bacterial and/or viral) pathogens were detected in 11% of the patients and in 2% of the controls (p = 0.028).
4 The etiological agents of diarrhea are numerous, including multiple viral, bacterial, and parasitic pathogens.
In this paper, we extend the TOFI pipeline to design microarray probes for multiple, related, bacterial and viral pathogens.
It obviates the need for an assumption to guild testing suspected viral pathogens with multiple viral-specific primers to amplify targets present in a sample, thus does not require prior sequence knowledge of the viral agent for primer design.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com