This indicates that multiple variant fusion transcripts use the same exon junctions.
Because Northern blot probes can also elucidate targets on multiple LHR splice variant transcripts, Northern analysis was also ruled out as a viable alternative method.
Due to alternative polyadenylation sites and variant transcripts that may be differently regulated, some genes are represented by multiple probesets in the design of U133Plus2 arrays.
Second, multiple probes for the same gene also showed divergent responses indicating either non-specific binding (cross-hybridization) or the presence of variant transcripts that targeted individual probes.
Over 75% of all differentially expressed transcripts were for protein coding mRNA and the remaining were mostly 'processed transcripts' (transcripts without an open reading frame) or 'retained introns', mapping to purported splicing variant transcripts.
They are significantly different from those in other tissues, and these variant transcripts encode distinct proteins.
Multiple variants of proteins or transcripts can be generated from a single gene via alternative splicing, thus increasing the complexity of proteome and transcriptome.
In the case of multiple transcript variants, among the transcripts annotated as protein coding, the one with the highest ribo-seq read density in control conditions was brought forward for differential expression analysis.
For genes with multiple transcript variants, the longest transcript was selected.
It should be noted that AKT1, FBXO21, PDE7B, and FBXO4 have multiple variants (full-length, partial length, or transcript variants) present on the array, hence the appearance of the protein kinase, RAC-alpha serine/threonine protein kinase (AKT1) twice in the top 10 proteins bound by the synbody.
