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Previously, we identified that DH012 infected animals mount potent neutralizing activity against a conformational epitope (CEV) that involves multiple variable regions.
These protein fragments are considered antibody-mimetic because, like antibodies, each protein fragment is composed of a constant region, which stabilizes the overall protein folding, and multiple variable regions that mediate its binding to a specific target.
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For example, Liepelt et al. (2001) noted that polymerase error can occur at a higher rate in regions containing multiple variable repeat regions.
The primers were designed, by multiple sequence alignment of variable regions, specific for cold-adapted donor virus HTCA-A101, as compared to other influenza A viruses.
Taken together, the results suggest that replication origins evolved multiple times in the variable regions of the chromosome and were continually being created and destroyed.
These sequences were aligned using Clustal Multiple Alignment to identify conserved and variable regions suitable for primers and probes that could differentiate the species.
In many cases, the size, number, and polarity of smaller indel events were ambiguous because multiple indels often overlapped in structurally variable regions.
Multiple unique heavy or unique light chain variable regions were amplified in 3 wells and in one of these cases two unique heavy and two unique light chain variable regions were amplified.
Previous studies have shown that student misconceptions about science can differ significantly based on multiple variables including geographical region, religious background, generation, gender, and age (Almquist and Cronin [1988]; Losh et al.[2003]; Morrison and Lederman [2003]; Palmer [1999]).
Multiple alignments were created using MUSCLE software [ 34] and the highly variable regions of these alignments were removed using Gblocks [ 35].
Results show that there are multiple copies of the TATAATATA motif present in each of the three Variable Regions, but humans contain a significantly larger number than present in the chimpanzee (Table 1).
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CEO of Professional Science Editing for Scientists @ prosciediting.com