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These peptides represented 1031 proteins, with 837 proteins covered by multiple unique peptides.
The expression of five isoforms was ruled out, because multiple unique peptides from these variants were lacking from the peptide mixture.
But since we used multiple unique peptides for protein identifications, the overall protein false discovery rate is much lower than the peptide false discovery rate [ 17, 21].
Multiple unique peptides mapping to a single gene provide compelling evidence for the expression of the product(s) encoded by that particular gene.
In peptide design for MRM, the similar difficulty appeared in selecting multiple unique peptides of AKRs due to the highly homologous amino acid sequences in AKR proteins.
Quantitative variations among the multiple unique peptides for any of the 66 kinases are relatively small, as manifested by an average relative standard deviation (RSD) of 12.6% (calculated on the basis of data shown in Table S3, Supporting Information).
Similar(54)
However, by demanding that proteins are identified with multiple high quality MS/MS spectra (with FPR <1%) and multiple unique peptide sequences in multiple independent preparations, many of these contaminations can be eliminated.
For K12, multiple unique peptide sequences are detected within upstream repeats and may correspond to kaposin isoforms B or C (unannotated [ 16, 17]); all detected K12 peptide sequences are in the same frame.
We describe the generation of multiple, unique small peptides nine to thirty-four acids acins in length which, when labeled with the radioisotope 32P, bind with vastly differing efficiencies to cell lines derived from different colon adenocarcinomas.
Multiple unique ShhN* peptides were found in all samples, demonstrating the capacity of 2 to label endogenous ShhN even at very low native levels in diverse pancreatic cancer cell lines.
Most importantly, combining the use of multiple proteases to generate proteolytically unique peptides with ion mobility separation now allows for a wider mass range of proteins to be quantified.
More suggestions(15)
multiple unique cases
multiple intracellular peptides
multiple unique traits
multiple unique haplotypes
multiple unique events
multiple unique paths
multiple unique sensations
multiple unique inserts
multiple homing peptides
multiple unique species
multiple unique probes
multiple unique transcripts
multiple unique miRNAs
multiple unique markers
multiple unique features
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