Sentence examples for multiple turnover from inspiring English sources
Both cycles proceed uncatalyzed at mild temperature and neutral pH, with one cycle demonstrating multiple turnover with sequential feeding of source materials (glyoxylate and H2O2).
Most significantly, this ribozyme is able to exhibit multiple turnover activity and charge parasubstituted Phe analogs onto an engineered suppressor tRNA (tRNAAsnCCCG).
Under a multiple turnover condition, both performed well at 37°C, showing the kcat of 1 and 0.26 min−1, respectively.
By appropriate engineering, a group I intron can be converted into a multiple turnover RNA enzyme, or ribozyme, capable of cleaving single-stranded RNA substrates.
Selections for enzymes can be performed indirectly (for binding of a transition-state analogue or mechanism-based inhibitor), and directly using either intramolecular single-turnover selections (e.g. with SELEX) or the normal (intermolecular, multiple turnover) mode of enzymatic reactions.
We also demonstrate how multiple turnover experiments can provide evidence for either "burst-phase" kinetics, which can reveal a slow product release step, or linear-phase kinetics, in which the chemical step can be rate-determining.
Hutvágner, G. & Zamore, P. D. A microRNA in a multiple-turnover RNAi enzyme complex.
But Sanchez did not compound the first mistake the way he did in five multiple-turnover games last season.
They take the advantages of both aptamers and DNAzymes, which can recognize specific targets with high selectivity and catalyze multiple-turnover reactions for signal amplification, respectively, and have shown their great promise in many analytical applications.
We identified 77 novel FTase substrates that exhibit multiple-turnover (MTO) reactivity within this library; our library also contained 85 peptides that can be farnesylated by FTase only under single-turnover (STO) conditions.
Multiple-turnover analysis yielded the following kinetic parameters: Vmax=7.3+/-0.5 nM/min, Km=2.1+/-0.6 mM, and kcat=1.5+/-0.4 kcat=1.5+/-0.4
