Positive controls included separate TMA slides containing multiple tumour and normal tissues for EGFR and pEGFR, sections from tumours with known marker expression for HER-2/neu and PTEN, ovarian cancer cell line A2780 for AKT, pAKT and ERK, and glioblastoma cell line U87 transfected with an EGFRvIII plasmid for EGFRvIII staining (Jungbluth et al, 2003).
Here, we report the first comprehensive, quantitative analysis of EPOR transcript levels in multiple tumour types compared to tissues of tumour origin.
In addition, simultaneous measurements of multiple kallikrein levels in tumour tissues may have more prognostic value than that of each individual kallikrein.
Thus, age-related methylation has the potential to behave as a mutator process resulting in the simultaneous silencing of multiple tumour related genes in aging tissues (Lipkin, 1988; Issa, 2000).
Therefore, although VEGF levels have been measured by multiple methods in tumour tissue and in serum/plasma, the relationship between tumour tissue and circulating VEGF is still unclear (Martins et al, 2011).
Oncomine expression microarray data were examined to determine the expression of NDN transcript in multiple tumour types compared with normal tissue.
This technique of EC quantification in tumour tissues was applicable for tumours of multiple types and origins.
To evaluate the importance of ID1 in malignant melanoma, tumour cell expression was examined by immunohistochemistry in 119 cases of nodular melanoma using tissue microarray technique, and related to multiple tumour markers including proliferation, p16 expression, angiogenesis and patient survival.
These results highlight the need to consider multiple distinct mechanical stimuli when modelling the force microenvironment of tumour tissues.
Imaging correlates for genetic markers would potentially provide an adjunct to histologic assessment by enabling simultaneous evaluation of the whole tumour and multiple tumour sites and by providing an indication of mutation status when tissue sampling has failed.
