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Hypoxic fractions ranging from 4 to 13% have been estimated on the basis of morphometric analysis of multiple tumour sections.
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Positive controls included separate TMA slides containing multiple tumour and normal tissues for EGFR and pEGFR, sections from tumours with known marker expression for HER-2/neu and PTEN, ovarian cancer cell line A2780 for AKT, pAKT and ERK, and glioblastoma cell line U87 transfected with an EGFRvIII plasmid for EGFRvIII staining (Jungbluth et al, 2003).
Three representative tumour sections are shown.
No staining was observed in DU145 tumour sections.
Analysis of tumour sections showed that TG2 was expressed mainly in the more viable rim of the tumours and less in the necrotic core of the tumours.
A biopsy-based approach to multiple tumour lesions has practical limitations.
Tumour sections included normal pancreatic tissue.
Representative tumour sections were stained for CD105.
Tumour sections were counterstained with haematoxylin.
Tumour sections were also subjected to H&E staining.
Tumour sections were inspected at × 20 and × 40 magnification.
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