In spite of limitations which are frequently encountered in cancer and other tissue studies in humans, such as a modest sample size, heterogeneous tissues, and multiple categorical variables (tumor stage, gender, and individual patient variation), we were able to identify and validate a number of candidate colon cancer-specific splicing events.
In the multivariate Cox regression analysis, only high KPNA2 expression (P = 0.001), male gender (P = 0.002), multiple tumor foci (P = 0.013) and tumor stage(p = 0.022) remained significant.
The treatment and prognosis for breast cancer patients depends on multiple tumor characteristics including the size, stage, extent of tumor delocalization, and expression of various protein and mRNA prognostic factors [ 35].
A univariate Cox regression analysis (Table 3) revealed that male sex (P = 0.004), tumors located in the ureter (P = 0.015), multiple tumor foci (P = 0.009),high stage(P = 0.012) and high KPNA2 expression (P < 0.001) were highly associated with a shorter DFS.
Furthermore, Fibulin-5 protein was expressed at significantly lower levels in HCC patients with multiple tumor nodes, venous infiltration, high Edmondson-Steiner grading and advanced TNM tumor stage.
Low expression of Fibulin-5 was significantly correlated with poor prognostic features including multiple tumor nodes, venous infiltration, high Edmondson-Steiner grading and advanced tumor-node-metastasis (TNM) tumor stage.
As shown in Table 1, low expression of Fibulin-5 protein was prominently associated with multiple tumor nodes (P = 0.001), venous infiltration (P = 0.011), high Edmondson-Steiner grading (P = 0.014) and advanced tumor-node-metastasis (TNM) tumor stage (P = 0.033).
In patients with multiple lesions, tissue sample was obtained from at least one tumor (the most advanced one or the largest when multiple tumors had the same tumor stage).
Tumor stages were based on the AJCC staging system.
