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When available, tissue blocks from multiple tumor sections were assessed.
NT not tested When available, XAGE-1b overexpression was assessed in multiple tumor sections XAGE-1b-specific IgG antibody response is shown In our patient cohort, the whole primary tumor was available for XAGE-1b staining from 28 patients who underwent surgical resection (stage I/II).
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The potential contribution to the H3T assay signal by non-specific Ab6 and B9A11 binding to fat and non-tumor stroma was evaluated by comparing H3T measurements obtained using multiple paired tumor sections that were or were not macro-dissected to remove non-tumor tissue prior to testing.
These studies followed GLP guidelines and employed Stratagene reference human RNA mix, pooled tissue homogenates derived from FFPE tissue sections and multiple sections of FFPE tumor sections from a variety of tumors.
However, this setup provides the possibility to study multiple parameters in entire tumor sections, which is difficult to achieve on a large scale in a patient setting.
TARS antibody staining was optimized for the greatest range of detection by testing multiple dilutions (1 50 1 300) using benign and Stage 3 ovarian tumor sections.
We evaluated the frequency of CUL3, RBX1, and KEAP1 disruption across multiple tumor types from the TCGA, selected based on data availability from TCGA (Section 2).
Processing of sections from the bowel tissue, for histology and H&E staining, revealed multiple tumor nodules in mesentery fat tissue that could develop from tumor cell migration through adipose tissue blood vessels.
To validate our pH2AX findings and to further address the presence of DNA damage or DNA damage response, we probed tumor sections with a primary 53BP1 antibody and counted the fraction of cells with multiple 53BP1 nuclear foci (Figure 3B).
(D) CD31 immunohistochemical staining on tumor sections.
Fig. 5 Photomicrographs of stained tumor sections.
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