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We performed multiple transgenic injections to investigate these variables and observed little difference in outcome (Table 2).
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As the first step to determine cellular sites of EAT-6 function in ACh signaling, we constructed a GFP reporter under the control of the eat-6 promoter (peat-6::gfp) and generated multiple transgenic lines from independent injections of this construct.
Multiple transgenic lines for each transgene were examined for fluorescence expression, rescue and/or localization patterns.
Multiple transgenic lines were established using pTurbo helper plasmids under standard injection procedure (Tanaka-Matakatsu and Du, 2008), and examined all lines to confirm the consistency.
Multiple transgenic lines carrying a UAS-Aβ42, UAS-Aβ42Arc, or UAS-Aβ42art transgene were established.
Note that GM Stacks can be obtained also by multiple transgenic modification.
If so, we do not know how this mechanism will cope with multiple transgenic inserts.
Multiple transgenic lines were generated and tested for each construct.
Transgenic injections were performed by BestGene Inc. (Chino Hills, CA).
The constructs were used to produce multiple transgenic lines.
Therefore, developing and testing multiple transgenic founders will be crucial to gain a transgenic mouse line with optimal expression patterns.
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