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Therefore, developing and testing multiple transgenic founders will be crucial to gain a transgenic mouse line with optimal expression patterns.
Multiple transgenic founders share expression in the cranial ganglia (CG) surrounding the eyes and the lips (L), the premaxilla (PM), under the jaw (J), and in armor plates (AP).
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The MCK-miR-133a-1 MCK-miR-133a-1 MCK-miR-133a-1 MCK-miR-133a-1lized mouse eggs and multransgenicsgeniconstruct lines wase obtainjected verintod by PCR genotyping.
Germline transmission of the transgenes was obtained in 5 transgenic founders for Tg-110 (from 6 founders) and two for Tg-250SB (from 4 founders).
This is consistent with the founder being mosaic for the transgene, which is not uncommon in transgenic founders.
Two transgenic founders harbouring the full-length transgene could be identified.
The first, based on lentivirus vectors, allows efficient generation of transgenic founders, most of them expressing the transgene.
In these three transgenic founders, the splice region of Δ16HER2 transgene was sequenced.
Monitoring luciferase expression pattern in live mice enables screening of large numbers of transgenic founders quickly and inexpensively.
Both tested liver-specific and developmentally regulated promoters exhibited the expected expression pattern in most transgenic founders.
Several transgenic founders with germ line transmission were obtained.
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