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Minichromosome technology provides one solution to the stable expression and maintenance of multiple transgenes in one genome.
The recent cloning and characterization of β-carotene ketolase genes in conjunction with the development of effective co-transformation strategies permitting facile co-integration of multiple transgenes in target plants provided essential resources and tools to produce ketocarotenoids in planta by genetic engineering.
The second feature, expressing multiple transgenes in the same individual neurons, has been very difficult to achieve.
For studying molecular mechanisms underlying structural plasticity, it is often required to express multiple transgenes in the same individual neurons.
Given the use of multiple transgenes in many crossing schemes, it is common to use two transgenes that have been independently inserted into the same integration site (attP site).
Integrating multiple transgenes in an array can compensate for a shortage of landing sites in systems or species where landing sites are scarce and isolating new sites is impractical.
Similar(53)
The hyperactive integrase activity of Int* can sequentially integrate multiple transgenes into a single landing site to create an array of transgenes linked in tandem.
The system enabled the repeated integration of multiple transgenes into a predetermined locus of a genome.
Earlier studies also demonstrated that gene silencing by stable integration can be triggered by the presence of multiple transgene copies in transgenic plants with varying degrees of target reduction (Waterhouse et al. 2001; Kerschen et al. 2004).
Relevant changes include the rapid increase in the number of candidate genes becoming available through various genome projects, ongoing improvements in the capacity of crop biotechnologists to insert multiple transgenes, and predicted decreases in the costs of the development and regulatory processes.
As multiple transgenes can be expressed in the same isolated neurons using the Cre-3487 tranimalsc anitalshouldshould be possible to express other transgenes together with EGFP-F from line TLG 1157.
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