Your English writing platform
Discover LudwigSuggestions(1)
Exact(1)
Multiple transformants with clrB expression cassette insertion and resistant to pyrithiamine were screened, and one of them was verified by PCR and Southern blot (Additional file 1: Figure S1A).
Similar(59)
Multiple transformants were identified by screening on plates containing 30 mg l-1 kanamycin.
The above patterns were observed in multiple transformants expressing either the N- and C-terminal GFP fusions.
Multiple transformants showed the same cell size phenotypes.
Multiple transformants were streaked onto SCM-Ura plates containing glucose or galactose.
We generated gene trap barley lines by crossing Ac transposase expressing plants with multiple independent transformants carrying the Ds based gene trap construct GT DsB.
Interestingly, the average number of plasmids born by multiple vector transformants did not vary with their library population proportion.
Particle bombardment can also be used to obtain transformants with variable success rates [ 8, 9], but can also introduce multiple copies that may recombine or result in co-suppression [ 10].
The quantitative information described herein should increase awareness of this issue, and the rapid sequencing approach developed for these studies should be widely useful for identifying multiple vector transformants and avoiding complications associated with cells that have acquired more than one unique plasmid.
Transformation of the alb1 mutant with pAUR-PksP yielded transformants with black conidia, whereas all transformants of alb1 with pAUR325 remained albino (Figure 3B).
(B) Pigmented colonies formed in transformants of alb1 with pAUR-pksP (left), but transformants with pAUR325 remained albino (right).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com