Exact(6)
Multiple transformants were identified by screening on plates containing 30 mg l-1 kanamycin.
The above patterns were observed in multiple transformants expressing either the N- and C-terminal GFP fusions.
Multiple transformants were identified by screening on plates containing 30 mg l−1 kanamycin and homozygous lines were obtained in T3 generation.
Multiple transformants showed the same cell size phenotypes.
Multiple transformants were streaked onto SCM-Ura plates containing glucose or galactose.
Multiple transformants with clrB expression cassette insertion and resistant to pyrithiamine were screened, and one of them was verified by PCR and Southern blot (Additional file 1: Figure S1A).
Similar(54)
Both vector concentration and vector to insert ratio influenced the library proportion of multiple vector transformants, but their population frequency was independent of transformation efficiency.
Multiple overexpression transformants were generated and PCR of the target genes were performed to confirm stable transformation.
In contrast to bacterial systems, there is little quantitative information available regarding multiple vector transformants in yeast.
Interestingly, the average number of plasmids born by multiple vector transformants did not vary with their library population proportion.
These results highlight the potential for multiple vector transformants to dominate yeast libraries constructed by homologous recombination.
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