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The transit plan was heavily criticized because athletes would have to navigate multiple transfers between buses, ferries and trains to get to their destinations.
The branching order between the eukaryotic GapA/B* genes is well supported, and at face value this result suggests that there were either multiple transfers between eukaryotes or that the gene originated in chromalveolates and was transferred to diplonemids.
However, some of these cell lines have been acquired following multiple transfers between laboratories, so authenticity and freedom from microbial contamination are not guaranteed unless specifically stated in a Certificate of Analysis.
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Effort should be made to minimise multiple transfers of CSF between surfaces, and record how many such steps a sample has gone through.
The intermingling of sequences from the UK and Brazil in phylogenies also raises the possibility that multiple transfers of subtype C viruses between the UK and Brazil (in both directions) may have occurred in recent times.
Information from the EFL phylogeny may provide clues for further distinction between either multiple transfers or ancient paralogy with subsequent losses.
Interestingly, the TE and host encoded domains are distributed throughout this clade suggesting the possibility of multiple transfers of THAP domain CDS between TE and host genomes.
These multiple transfers also resulted in the discrepancy between the totals of 'received' and 'donated' lice.
If multiple transfers are found in each path between these confidence intervals, the shortest path is considered (Additional file 1: Figure S2e).
This is a case where sequence phylogeny might help, but it sometimes yields trees where it is very hard to distinguish between multiple losses and multiple transfers.
In a sprawling system in which riders can face multiple transfers to get to their destinations, the design creates a rare opportunity for riders: a seamless, 22-mile trip between Norwalk and the Mid-City area of Los Angeles.
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