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Multiple transcripts were analyzed simultaneously for 40 cycles using an optimized qRT-PCR thermal profile.
Interestingly, when multiple transcripts were detected for a CG, alternative splicing was observed in 61% of the cases.
Reads that had equal-quality matches to multiple transcripts were excluded.
Reads aligned to multiple transcripts were not considered for estimating the depth of the transcript.
Indeed, multiple transcripts were produced from a gene by utilizing variable TSSs.
Functional annotates acquired from the list of regulated genes and the probe sets that identified multiple transcripts were removed.
Similar(41)
Instead, multiple transcripts are expressed at levels within the range of that observed within species.
To create exon skip junctions in silico, a gene containing multiple transcripts was first reduced to a single 'full length transcript' (Fig. 2a) as described in Materials and Methods.
Through alternative splicing, multiple transcripts are produced from a single mRNA precursor, widely expanding proteome diversity.
Among ncRNAs, the existence of multiple transcripts is relatively common for eukaryotic miRNAs and their precursors [ 42].
For simplicity, multiple transcripts are present at the same concentration in this modified design, rather than each transcript at a different concentration.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com