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For this we created a diminished non-redundant set of 13,216 base genes from the total set of 14,078 by eliminating multiple transcripts representing the same gene (see methods).
Finally, the longest splicing variant was chosen when multiple transcripts representing the same gene were annotated if not otherwise specified.
For example, multiple transcripts representing cholesterol-homeostasis genes including HMGCS1, HMGCR, IDI1, FDPS, FDFT1, NSDHL, EBP, DHCR7, INSIG1, FADS1 were upregulated by P4 exposure (Additional File 2).
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It was often necessary to evaluate the co-expression between gene symbols or Unigene identifiers for which there were multiple transcripts represented on the Affymetrix 430 2.0 array.
Probe sets targeting multiple transcripts represented by suffix (_x_ at, _g_at, _f_at,_r_at,_b_at,_l_at and _i_at) were filtered out and only probe sets with higher specificity represented by suffix (_at and _s_at) were taken into consideration for further analysis.
All results therefore suggest that the Mito1 transcripts represent polyadenylated transcripts of the mitochondrial rpl2 gene.
The remaining 34 transcripts represent 32 genes.
In contrast, the process of SAGE follows the approach of multiple transcripts-multiple tags-single clone-single sequencing; thus, each SAGE sequence represents multiple transcripts.
Every enzyme in the pathway was represented by multiple transcripts except for 4-coumaroyl CoA ligase and anthocyanidin synthase, each of which was represented by a single EST.
Both proteins are encoded by multiple gene copies each represented by multiple transcripts in the K. brevis EST data.
Both genes are represented by multiple transcripts and have a GC content typical for K. brevis nuclear encoded genes (Table 2).
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CEO of Professional Science Editing for Scientists @ prosciediting.com