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Complications arise due to complexity of the IGF-I system, since multiple transcripts of the IGF-I gene encode different isoforms generated by alternate promoter usage, differential splicing and post-translational modification, and how IGF-I gene expression relates to its diverse autocrine, paracrine and endocrine modes of action in vivo has still to be elucidated.
Furthermore, any length normalization is imperfect because so many annotated loci have multiple transcripts of different lengths.
The XML file was then processed with Perl scripts to eliminate non-melanogaster sequences, to group multiple transcripts of the same gene, and to evaluate the regular expression /P[AGVLIPFMWCNQSTWY]GP/ which identified sequences with putative collagenase restriction sites.
Determining transcriptional initiation and termination sites for each miRNA will allow to determine if the multiple transcripts of miRNAs are produced from overlapping genes or polycistronic effect may play a role.
Multiple transcripts of IGF2 are produced as a result of alternate promoter usage and splicing: promoter P2-P4-dependent transcripts do not contain exons 1 3, which are incorporated in P1 promoter-dependent counterparts.
If there were multiple transcripts of the same length, one was arbitrarily chosen.
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It also appears that like in mouse and human, there may be multiple transcripts for some of the tammar IFITM genes.
This decision is justified by the biological existence, especially for mammalians, of multiple transcripts for each gene, each with a potentially different tissue expression pattern.
Multiple transcripts composed of different exons due to alternative splicing of FPGT-TNNI3K readthrough transcript have been described in human [ 37].
The bbg gene locus spans over 120 kb and encodes multiple transcripts (5 of the 8 are shown; Figure 3A) (Kim et al. 2006).
On the cellular level, chromosomal region-specific demethylation may open for transcription of multiple transcripts from the same locus, which then could lead to the observed presence of multiple overlapping transcripts.
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