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If there are multiple transcripts for a gene, the transcription start site (TSS) and 3' UTR of the major transcript were used [ 23].
These included 29 transcripts for WRKY domain proteins, and multiple transcripts for PTI5, RAV, NAC and MYB-like transcription factors, some of which are known to be transcriptional regulators of various disease responses [ 48- 50].
Multiple transcripts for each gene have been cloned by reverse transcription-PCR from human brain, spinal cord and testis cDNA libraries.
Northern analysis on adult tissues (Figure 7B) revealed high levels and multiple transcripts for Irm RNA in the brain.
If multiple transcripts for the same gene met these criteria, we selected the one with the highest Pol IITSS signal (breaking ties using the mean Pol IITU enrichment).
This decision is justified by the biological existence, especially for mammalians, of multiple transcripts for each gene, each with a potentially different tissue expression pattern.
Similar(47)
The analyses of ESTs provides some of the most detailed data for multiple transcripts from a single gene, particularly evidence for monofunctional LKR transcripts in switchgrass and monofunctional SDH transcripts in wheat and Brachypodium.
Most R/Bioconductor microarray workflows function only at the gene level, by associating a single gene with a set of probes and calculating the average expression for multiple transcripts of the same gene.
Throughout the paper, the number of genes identified is reported, while the Additional files contain information on the specific transcripts for each gene, including multiple transcripts identified for the same gene.
Where multiple transcripts exist for a gene, we selected the longest transcript as the representative transcript for the gene in the transcriptome.
When there were multiple transcripts available for genes, only the primary transcript was assessed.
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