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We found that the probe sequences on the HG-U133A were sufficient to distinguish multiple transcript intensities for 215 genes (or 141 genes with a minimum probe set size of 3).
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Furthermore, if a gene encodes multiple transcript isoforms, the gene could be revealed by plotting the probe signal intensities against the gene structure [28], [29].
For genes with multiple transcript isoforms, only the first named or numbered transcript was retained.
Alternative splicing of this gene results in multiple transcript variants.
Redundancies due to annotation of multiple transcript isoforms were filtered.
Ten variant exons in this gene generate multiple transcript variants.
For genes with multiple transcript variants, the longest transcript was selected.
Only Method 4 (no depletion_NuGEN) had a significant portion of uniquely detected transcripts with probe set signal intensities above the lowest quartile of detected transcript intensities.
Alternatively, these probes may contain conserved motifs and therefore the probes on the microarray will cross-hybridize to multiple transcripts resulting in the higher signal intensities observed on the microarray for these elements.
To compare the expression patterns of two multiple transcripts derived from DSP-PP gene during tooth development.
It is controlled by two promoters and has multiple transcripts.
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