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Detection of multiple toxic mycotoxins is of importance in food quality control.
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Aflatoxins are among the most toxic mycotoxins.
In conclusion, by combining the toxic-response gene SERPINB2 and human stem cells, we can develop an in vitro screening platform for multiple toxic substances.
Recently, a multiplex dipstick immunoassay for the indirect detection of ZEN, deoxynivalenol (DON), T-2/HT-2 toxin and FB was developed, but omitting the most toxic mycotoxins.
It is unlikely that single end point hormesis would be quantitatively useful for toxics expressing multiple toxic effects in multiple organs and through multiple mechanisms.
Aflatoxins are highly toxic mycotoxin contamination, which pose serious food safety incidents.
The selective detection of ultratrace amounts of aflatoxin M1 (AFM1) is extremely important for food safety since it is the most toxic mycotoxin class that is allowed to be present on cow milk with strictly low regulatory levels.
In this study, S. mycoparasitica SMCD 2220-01 was efficient in decreasing the level of multiple Fusarium mycotoxins including ZEN by 97%, DON by 89%, 15-ADON by 72%, and 3-ADON by 58% revealed by TLC and HPLC ESI HRMS.
LC-MS-based methods can render multiple native mycotoxins and potentially also conjugated forms directly accessible for detection [ 22].
Using conventional surface plasmon resonance (SPR), several applications are known for single [ 28] and for multiple (4) mycotoxins [ 29].
Currently there is an urgent need for multi-mycotoxin detection methods due to the co-occurrence of multiple mycotoxins in food raw materials and their augmented toxicity.
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