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An appropriate control gene for comparing human and chimpanzee expression across multiple tissues was defined by steady expression between and within species as well as across tissues.
Thus, the expression level across multiple tissues was explored for both transcripts.
The expression pattern of SAMD9 in multiple tissues was investigated using a human multiple tissue cDNA panel (Clontech, USA) and RT-PCR.
5′RACE cDNA from multiple tissues was prepared from 200 to 500 ng of total RNA with use of the BD SMART RACE cDNA amplification kit (Clontech).
In this study, the longest isoform, which is the major isoform constitutively expressed in multiple tissues, was used for functional assays.
It is possible that other tissues, for example gills, might have shown a greater response to hyposmotic challenge, but measurement of expression changes in multiple tissues was outside the scope of this study.
Similar(53)
When multiple tissues are submitted and aerobic culture is requested, we will culture each separately, and a fee for each tissue will accrue.
While phylogenetic analysis of transcriptomes of the same tissue is usually congruent with the species tree, the controversy emerges when multiple tissues are included, that is, whether species from the same tissue are clustered together, or different tissues from the same species are clustered together.
Multiple tissues were dissected from the mice.
The multiple tissues were used for the isolation of total RNAs.
Moreover, the mRNA expression patterns of CDT2 and INTS7 in multiple tissues were inconsistent.
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