Human or mouse multiple tissue cDNA panels (Clontech) were probed using oligonucleotide primers, according to the manufacturer's instructions.
Expression of SAMD9 and its paralogous gene SAMD9L were investigated using PCR of human multiple tissue cDNA panels including adult, fetal and tumor tissues (Clontech, USA).
RNA samples from all additional human tissues and cDNA from purified blood fractions (Multiple Tissue cDNA panels) were purchased from Clontech.
The tissue-specific expression of the two transcripts of the APITD1 gene was analysed by amplification of commercially available cDNA from CLONTEC Hs Multiple Tissue cDNA Panels (CLONTECH, Palo Alto, CA, USA) and from OriGene (OriGene Technologies, Inc., Rockville, MD, USA), using two primer pairs.
Quantitative real time PCR was used to screen a mouse multiple tissue cDNA panel (Clontech, Mountain View, CA, USA) and RNAs isolated from the inner ear of P0 mouse for the presence of otolin transcripts.
The expression of PCDH15 mRNA was analyzed using the human multiple tissue cDNA panel 1 (Clontech).
Next, we investigated the tissue distribution of PCDH15 in different human tissues using a commercial human multiple tissue cDNA panel of eight different tissues.
The expression pattern of SAMD9 in multiple tissues was investigated using a human multiple tissue cDNA panel (Clontech, USA) and RT-PCR.
Siglec-H expression was performed using a Mouse Multiple Tissue cDNA Panel I (BD Bioscience Clontech) as well as WT brain at P1 and P4.
To determine the levels of expression of human VPS25 in different cell types, we screened a human multiple tissue cDNA panel.
