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In multiple tissue culture assays of angiogenesis the exposure of endothelial cells to EPC-CM resulted in increased proliferation, migration and organization of the cells into cord structures.
Rotarix® was developed by multiple tissue culture passages of the human G1P[8] parental 89 12 strain.
The protocol described here is easy to implement and should facilitate high-titer LV vector production for preclinical studies in animal models without the need for multiple tissue culture dishes and ultracentrifugation-based concentration protocols.
These protocols are easy to implement in a standard laboratory setting and allow high-titer LV vector production without the need for multiple tissue culture dishes or flasks or ultracentrifugation-based concentration procedures.
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Swabs as well as multiple tissue cultures were taken.
The joint was opened using a posterolateral approach and multiple tissue cultures were taken from the joint fluid, (pseudo-) capsule, and membranous tissue at the interface of bone and implant/cement.
The hip joint was opened using either a direct lateral or a posterolateral approach (25 vs. 8, depending on the approach at index surgery) and multiple tissue cultures were taken from the joint fluid, (pseudo-) capsule, and membranous tissue at the interface of bone and implant/cement.
Primary astrocytes and U-87 astrocytoma cells were cultured as described above in multiple 150 cm2 tissue culture flasks, harvested, washed in sterile RNase-free ice-cold phosphate buffered saline (PBS: Ambion, Streetsville, ON, Canada), pooled and pelleted by centrifugation at 196 g for 5 min at 4°C as previously described in detail [46].
To measure clonogenicity, U251 cells at a given cell density were plated onto multiple well tissue culture dishes, with and without addition of increasingdoses of CAERS or CFEZO.
These stained with antibodies to mesenchymal elements (collagen I, vimentin, αSMA and BMP receptors), and maintained this phenotype through multiple passages in tissue culture [ 89].
APC has multiple effects in tissue culture systems, including limitation in NF-κB-mediated proinflammatory activity [ 110], attenuation of inflammatory cytokine and chemokine generation [ 23], and upregulation of anti-apoptotic genes of the Bcl-2 family of homologs [ 111].
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