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Thus, while multiple theoretical peptides may fall close in mass to a given precursor ion, the correct peptide sequence will almost always match the MS2 spectrum with a significantly higher score than competing, incorrect peptides.
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Before analyzing the results, we briefly investigate the possibility of different peptides sharing multiple theoretical peaks in our test data.
Certainly, multiple theoretical perspectives can be used to glean insights.
At the peptide level, peptides were excluded if the ratio of observed to theoretical peptides was <0.2, when the theoretical was less than 100, or a ratio of <0.1, when the theoretical value was greater than or equal to 100.
The peptide masses were matched to theoretical peptides generated from the precursor sequence.
Trypsin specificity with 3 missed cleavages was used to generate theoretical peptides.
Over the past decades, technology acceptance has been researched from multiple theoretical perspectives [ 7].
There are multiple theoretical, methodological, policy and practice contributions of this research.
Our full questionnaires operationalised multiple theoretical models that resulted in long questionnaires asking seemingly repetitive questions.
This is one type of vulnerability assessment, for which multiple theoretical frameworks and methodologies are available.
In the next step we calculated an optimal theoretical peptide elution rate, Q = N/ TΔ, where N is the number of theoretical peptides and TΔ is the gradient time.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com