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Most dendrochronological studies of genetic variation in tree species have examined multiple provenances at a single test site (e.g., Eillman et al. 2014), but our study is one of only a few that have examined radial growth sensitivity of multiple provenances established at multiple test sites.
The national examination will be a 12-station audiovisual recorded OSCE held in multiple test sites.
These results demonstrate that the Prosigna assay is analytically reproducible even when performed at multiple test sites and including all process variables.
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One large study involving multiple testing sites in three US states found increased detection yields of between 1.4 and 5.9% (and an overall prevalence of AHI of 0.3 per 1000 persons tested) [26].
This is important as the choice of an appropriate scoring system for a specific combination of chart type and viewing distance is required for accurate measurements that are repeatable across multiple testing sites and testers.
The precision and reproducibility of the Prosigna assay, estimated from repeat measurements of pooled tumor RNA sample(s) and de-identified patient tissue samples across multiple testing sites is similar (relative to the overall test range) to what was previously reported for centralized lab tests [ 32, 38].
Both providers and campaign-testers attribute this success to several factors: the availability of multiple testing sites, the fact that testing is free during campaigns (which is not always the case outside campaigns) and the information and awareness component of the national campaign.
In a multiple test-site study, the 3-D model gave an 80% overall concordance with rabbit irritancy data with 100% sensitivity [ 16].
Recently, the MicroArray Quality Control (MAQC) consortium addressed the reliability of data obtained using microarrays, by directly comparing performance across multiple platforms, test sites and replicates [ 19].
In order to estimate the number of expected false positives due to multiple testing of sites, a false discovery rate (FDR) was calculated for each model using 1,095 random SNPs throughout the genome.
We also found 82 unigenes (37 after multiple test correction) with sites evolving under positive selection in the ancestral lineage before the split between nine- and three-spined sticklebacks (Additional file 3: Table S4).
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