Sentence examples for multiple temperature reverse from inspiring English sources

RNA was dissolved in 25 µl of 5 mM Tris/0.5 mM EDTA and 9.5 µl RNA was used for the reverse transcription by AffinityScript Multiple Temperature Reverse Transcriptase (Stratagene) with random primers (Invitrogen) as described by the manufacturer.

cDNA was synthesised from 1 μg of the purified total RNA using 300 ng random primers (Promega) and the Stratagene AffinityScript™ Multiple Temperature Reverse kit in 20 μl reaction.

For real-time quantitative PCR (qPCR), total RNA was isolated by RNeasy Mini kit (Qiagen, Hilden, Germany) from mDF and used as template for complimentary DNA (cDNA) synthesis (AffinityScript multiple temperature reverse transcriptase, Stratagene).

The highest ratios were observed by the SuperScript® III Reverse Transcriptase and AffinityScript Multiple Temperature cDNA Synthesis kit, both achieving a score of 6.

For the quantification of NMD target gene expression, total RNA was isolated and cDNA was synthesized using the Affinity Script Multiple Temperature cDNA Synthesis kit or with SuperScript® III Reverse Transcriptase (Invitrogen, Darmstadt, Germany).

Total RNA extraction from the MGE of E13.5 wild-type and Rac3 knockout embryos was performed with Trizol (Invitrogen), and followed by cDNA reverse transcription using the Affinity Script Multiple Temperature cDNA Synthesis Kit (Agilent), according to the manufacturer's instructions.

One microgram of DNase-digested total RNA was reverse-transcribed to first strand complementary DNA (cDNA) using AffinityScript Multiple Temperature cDNA synthesis kit (Agilent, Edinburgh, UK) according to manufacturer's instructions.

Briefly, total RNA was reverse transcribed following a first-strand cDNA synthesis protocol from an AffinityScript Multiple Temperature cDNA synthesis kit (Agilent, Santa Clara, CA, USA) and amplified using an iProof High-Fidelity PCR kit (Biorad, Hercules, CA, USA).

A statistical analysis of multiple temperature proxies: are reconstructions of surface temperatures over the last 1000 years reliable?

The temperature reverse mode is slightly observed between +700 and −200 masl.

It means investing in a cooler that provides water of multiple temperatures.