Sentence examples for multiple temperature cDNA from inspiring English sources
The highest ratios were observed by the SuperScript® III Reverse Transcriptase and AffinityScript Multiple Temperature cDNA Synthesis kit, both achieving a score of 6.
cDNA was synthesized from 100 ng of RNA from each sample, using the AffinityScript Multiple Temperature cDNA Synthesis kit (Agilent Technologies).
Total RNA extraction from the MGE of E13.5 wild-type and Rac3 knockout embryos was performed with Trizol (Invitrogen), and followed by cDNA reverse transcription using the Affinity Script Multiple Temperature cDNA Synthesis Kit (Agilent), according to the manufacturer's instructions.
For the generation of the full-length Smg6 cDNA, total RNAs were isolated from E14.1 ESCs and cDNA was synthesized using an AffinityScript Multiple Temperature cDNA Synthesis kit (Agilent Technologies, Santa Clara, CA, USA).
Briefly, total RNA was reverse transcribed following a first-strand cDNA synthesis protocol from an AffinityScript Multiple Temperature cDNA synthesis kit (Agilent, Santa Clara, CA, USA) and amplified using an iProof High-Fidelity PCR kit (Biorad, Hercules, CA, USA).
One microgram of DNase-digested total RNA was reverse-transcribed to first strand complementary DNA (cDNA) using AffinityScript Multiple Temperature cDNA synthesis kit (Agilent, Edinburgh, UK) according to manufacturer's instructions.
For real-time quantitative PCR (qPCR), total RNA was isolated by RNeasy Mini kit (Qiagen, Hilden, Germany) from mDF and used as template for complimentary DNA (cDNA) synthesis (AffinityScript multiple temperature reverse transcriptase, Stratagene).
A statistical analysis of multiple temperature proxies: are reconstructions of surface temperatures over the last 1000 years reliable?
This week, Amazon posted a note on its site revealing that there were "multiple" temperature problems at the warehouse -- "low temperature events" as well as "high temperature events".
cDNA was synthesised from 1 μg of the purified total RNA using 300 ng random primers (Promega) and the Stratagene AffinityScript™ Multiple Temperature Reverse kit in 20 μl reaction.
The same sake can be served at multiple temperatures — cold, room temperature or warm — for three entirely different taste experiences.
