The pipeline clearly identified one BWS-HIL patient with large abnormal DNA methylation variations (may be due to technical variations) in multiple locations, though these may be due to technical variation (Additional file 1: Table S1).
In conclusion, these findings show that FMD-R is highly variable within subjects, even in a healthy population, after adjusting for multiple technical factors and implies biologic variation in radial artery tone.
To avoid potential technical variations between probes, the methylation levels of multiple probes for a single gene (average of four probes per gene) were not collapsed or averaged, and genes having multiple discordant probe intensities were eliminated from the analysis.
Floating foundation design poses multiple technical challenges.
That allowed an optimal control over potential technical variations.
Several technical variations could possibly contribute to this wide variation in the observed CT expression frequency.
This result is likely due to one or more of the technical variations which they introduced.
We observed minimal technical variations among the technical replicates of each sample.
All samples were coprocessed to eliminate technical variations.
At each step, technical variations and accompanying biases are introduced.
