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We addressed the first two points by performing multiple technical replicates of each subpool, and by checking the distribution of ancestry-informative SNPs through principal component analysis [24].
The mixed effect model accounted for potential correlation in signal intensities that may arise from having multiple brain regions from the same subject as well as having multiple technical replicates for the same brain regions.
Data was filtered for those showing expression on at least one array and a variance-stabilizing transformation [19] was done to take advantage of the multiple technical replicates (on average 20 30 per array) for each gene available on each Illumina chip.
At least two sets of five flies from each genotype were assayed, with multiple technical replicates.
for both WES and WGS across multiple technical replicates of five different reference samples.
Our evaluation focused on SNV and small indel detection for a single workflow across multiple technical replicates.
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This indicates a fundamental failure of the general SIEVE method to detect and analyze many of the peptides, even though many peptides had multiple annotated MS/MS spectra from multiple technical replicate samples.
Besides displaying expression profile from multiple biological or technical replicates within an experiment, the average expression of replicates can also be generated when checking "Show Average" checkbox.
Expression levels of all statistically significant and differentially expressed mRNAs and miRNAs were confirmed by qRT-PCR using TaqMan® expression kits (Applied Biosystems) [65] using multiple technical and biological replicates.
This is demonstrated in the next section, where we used real biological data with multiple biological and technical replicates.
Twenty samples with high missingness were re-run and an additional 29 samples passing QC were run multiple times as technical replicates.
More suggestions(15)
multiple technical ecosystems
multiple technical responses
multiple technical degrees
multiple technical variations
multiple technical failures
multiple technical areas
multiple technical issues
multiple technical guidelines
multiple technical solutions
multiple technical paths
multiple technical approaches
multiple technical examinations
multiple technical factors
multiple technical teams
multiple technical steps
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