If there are multiple targets involved, then the work becomes even harder.
By testing a small set of miR-181 targets in the reprogramming, Judson et al. then showed that miR-181, much like miR-294/302, represses multiple targets involved in the EMT, cell death, cell cycle, and mitochondria function.
The single target docking cannot capture the biological effects of the ligands comprehensively, and the multi-target docking is really necessary to characterize the complicated binding process of ligands with multiple targets involved in biological network.
These events were associated with TGFBR3-regulated multiple targets involved in CNE-2Z proliferation.
Numerous miRNAs are dysregulated in cancers, and a single miRNA can have multiple targets involved in different oncogenic pathways.
We examined the effect of drug exposure on glutathione- S-transferase-mediated oxaliplatin detoxification, DNA platinum adducts formation, cell cycle distribution, apoptosis, and the expression of multiple targets involved in DNA replication, recombination, and repair.
Reverse transcription PCR, western blot and activity assays were performed to assess whether enzastaurin influenced thymidylate synthase (TS) and the expression of multiple targets involved in cancer signaling and cell cycle distribution.
This complexity is due in part to the intricate biology of reproduction, the multiple targets involved (male, female, and offspring), the uncertainties in extrapolating from animal models to humans, and the problems involved in accurately characterizing exposures and outcomes in epidemiologic investigations.
Corresponding to the complexity of the components in diverse herbs or even in one herb, herbal medicines hit multiple biological targets involved in various pathogenesis.
The data presented here suggest that there are multiple circadian targets involved in regulating the day-night difference in urination behavior, including the bladder itself.
