Sentence examples for multiple target sequences from inspiring English sources
The multiplex ligation-dependent probe amplification (MLPA) technique allows simultaneous screening of multiple target sequences in a single reaction by using pairs of probes that carry tails for binding of common amplification primers.
Retroviral knockdown of miR-132 using a specific 'sponge' containing multiple target sequences impaired the integration of newborn neurons into the excitatory synaptic circuitry of the adult brain.
Multiple target sequences along with different combinations of promoters were screened to identify the most effective siRNA capable of gene knockdown in vitro and in vivo.
The MLPA technique allows simultaneous screening of multiple target sequences in a single reaction by using pairs of probes that carry tails for binding of common amplification primers.
In addition, the SYBR Green fluorogenic molecules is less expensive, simple in primer design and its universal RT-PCR protocols are suitable for multiple target sequences than the 5'-nuclease TaqMan assay.
Cooperative binding (19) of regulatory proteins to these multiple target sequences is essential for the precise and economical regulation of the recruitment of the transcription and/or replication machineries (20).
Multiple targeting sequences need to be used and the extent of downregulation at both the protein and RNA levels needs to be shown to reduce the possibility of off-target effects and to further validate the conclusions.
Therefore, multiple target KSHV sequences increase the detection efficiency, while nested PCR protocols are likely to introduce false positivity.
These results also demonstrate the non-interference of similar assay targets for RPM-Flu detection and identification of multiple target gene sequences from the same specimen.
Multiple target DNA sequences were detected with no statistically significant change in the observed CT values compared with the CT values observed for single-target detection (Table 2).
Here, we use a similar reporter vector sensitive to differentiation specific miRNAs to show that a single vector bearing multiple miRNA target sequences conjugated to different reporters can be used to monitor hiPSC formation from human fibroblasts and subsequent differentiation of the hiPSC.
