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The new library produced binding proteins (termed "monobodies") to multiple target proteins, generally with similar efficacy as the original, loop-focused library.
The decreased proteins such as HSP60, nucleolin, PPase, peroxiredoxin-4 and the increased protein (mtSSB) were identified in SKOV-3 cells after PC treatment, indicating that the effects of PC on tumor cell apoptosis may be relate to multiple target proteins.
The data also support the hypothesis that calmodulin contains multiple sites for protein-protein interaction that are differentially recognized by its multiple target proteins.
The function of the Spsb family is entirely unknown [28], therefore further research should be directed to scrutinize its biological function in the muscular activity of the oriental river prawn since Spsb proteins may regulate multiple target proteins [29].
Activation of p38 is mediated by multiple upstream kinases and this pathway plays a key role in the cell's stress response as well as the phosphorylation of multiple target proteins including phospholipase A2 and MAP tau.
To do so, Lys63-linked polyubiquitin has to specifically recognize multiple target proteins.
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It may also support the hypothesis that multiple target-protein binding sites exist in the αB-crystallin sequence, which are affected to a greater or lesser degree by conformational change.
Calcium signaling is critical for activation of T lymphocytes and has been proposed to be transduced through multiple calmodulin target proteins.
p53 is also essential for the responses to TGFβ signals by promoting the activation of multiple TGFβ target proteins (Cordenonsi et al., 2003; Termen et al., 2013).
There are several possible reasons for this difference: firstly, SB203580 may target more than one kinase involved in cell cycle arrest [16]; secondly, the stress-induced growth arrest via p38 may operate though multiple p38 target proteins; thirdly, it may be that the therapeutic window for MK2.III is too small, since it soon becomes inhibitory to cellular growth above its optimal concentration.
Depending on the source, these homogenized tissue samples have variable expressions of multiple cocaine target proteins.
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