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The microplate assay provides a useful tool to quickly screen large sample sets for multiple target organisms.
Laboratory-based methods may still prove to be cost effective if multiple target organisms can be detected cheaply.
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These discharges contain a mixture of organic and inorganic contaminants that may cause multiple biological effects (e.g., estrogenic, genotoxic, cytotoxic, or carcinogenic effects) due to interactions with multiple target sites in the exposed organisms [2, 3].
Furthermore, the number of cases documenting multiple resistance are increasing in frequency and involve a broad range of target organisms, including; viruses [ 1], bacteria [ 2], fungi [ 3], plants [ 4], and insects [ 5].
In PCR-based applications of NGS technologies amplicons are generated from target genes, often from multiple template genomes, and are sequenced in parallel without the need for separating target organisms or their DNA through sample sorting or cloning [ 1].
MicroRNAs (miRNAs) are a novel class of non-coding RNAs with lengths of 17 25 nucleotides (nt) that can regulate gene expression in eukaryotic organisms by pairing with target mRNAs to repress translation or cause degradation of multiple target mRNAs [ 4].
Multiple Target Ligand Database.
Multiple Target Ligands.
The target organisms were chosen based on their retention ratios.
Pharmaceuticals are designed to have physiological effects on target organisms.
As a pesticide, risks to target organisms in the agricultural field are accepted, but risks to non-target organisms, especially outside the areas of application, are considered undesirable.
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