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miRNAs bind to the 3'-untranslated region (UTR) of multiple target mRNAs and either block the target translation or initiate the target degradation [10], [11], [12].
The large number of miRNA genes combined with the potential of each miRNA to regulate multiple target mRNAs, implies a regulatory network of great complexity.
However, it has been well reported that small RNAs can be effective against single and multiple target mRNAs with incomplete complementarity [34], [35].
Each miRNA is predicted to recognize multiple target mRNAs [9], hence a single miRNA can have far-reaching effects on gene expression and may modulate the expression of gene sets that function together to control biological processes.
On the other hand, one miRNA usually inhibits multiple target mRNAs [ 9, 10].
In line with this, we find that RBM47 binds to multiple target mRNAs.
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5, 6 Single miRNA binds to multiple target mRNA, and regulates various cellular functions including proliferation, differentiation and metastasis.
The specific roles of individual miRNAs in the lens require comprehensive functional studies of their multiple predicted target mRNAs.
It is well documented that miRNAs generally possess multiple downstream target mRNAs, with some individual miRNAs controlling over 100 mRNAs [6].
Each miRNA has multiple different target mRNAs and each mRNA is regulated by several different miRNAs causing a very complex web of tight regulation.
We used a custom-made RT2 Profiler PCR Array (SABiosciences, Frederick, MD, USA), which consisted of a set of SYBR green fluorescent dye and multiple pre-designed primers with an arrangement to analyze multiple target gene mRNA expressions of experimental and control samples simultaneously.
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