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The structures of many cell surface adhesion proteins comprise multiple tandem repeats of structurally similar domains.
The second had multiple tandem repeats of a tryptic phosphopeptide sequence to give a similar increase in efficiency.
Our data demonstrates that artificial multiple tandem human IgA affibodies with relevant biological binding avidity were successfully yielded by phage-based molecular evolution.
Multiple tandem repeats stack in a linear manner to produce an elongated structure that is stabilized predominantly by short-range interactions between residues close in sequence.
The transcription activator-like (TAL) effector targets specific host promoter through its central DNA-binding domain, which comprises multiple tandem repeats (TALE repeats).
Earlier, we reported the design and characterization of a series of consensus TPR (CTPR) proteins, which are built as arrays of multiple tandem copies of a 34 amino acid consensus sequence.
Here, we report the generation of multiple tandem IgA affibodies by directed evolution using a combinatorial phage library displaying the IgA affibody A1 and/or A2 linked with three random amino acids.
The method is based on our previous finding that after transformation, DNA with homology to F. graminearum DNA recombines itself into the genome in a predictable manner involving multiple tandem copies.
Typical satellite sequences, consisting of multiple tandem repeats, were not identified.
Most of the unmapped sequences were contained in 1,696 short metacontigs and singleton contigs with multiple tandem repetitive sequences.
Frost contains multiple tandem repeats rich in serine, threonine and proline [9], a typical feature of mucins [31].
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