Enzymes were expressed in S. cerevisiae strains that contain either an overexpression plasmid with the open reading frame of interest, which was under control of the GAL1 inducible promoter and in fusion to a multiple tag at the C-terminus (Yeast ORF Collection, [34]) and N-terminus (Yeast GST-Tagged Collection [35]) or a chromosomally integrated gene with a tag fusion (TAP collection [36]).
Therefore, a signal collision caused by tag response can only be occurred at a given timeslot when multiple tags send their replies at the same timeslot.
To identify FPs with sufficient spectral separation to allow multiple tagging and, at the same time, minimize the requirement for computational separation of the resulting fluorescent signals we considered the relative biophysical and spectral properties of the FPs available [ 23, 24].
If multiple tags respond simultaneously, a collision at the air interface occurs.
Since the tags typically reply over the shared wireless medium and multiple tags can reply simultaneously to the reader, tag collision may occur at the reader.
Several had multiple tags.
Some have multiple tags, including the former chairman Peter S. Kalikow, a millionaire with dozens of cars, who reportedly has eight tags.
Mitty has multiple tags of comedy, fantasy, drama and romance.
For example, you can apply only one tag to each item ("Tech," say), not multiple tags ("Tech" and "Ridiculous").
