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Next, multiple substrates reactions were decomposed into components (panel C and D in Fig. 8).
Most members of the DHA12 family recognize multiple substrates and trigger conformational change upon substrate binding.
Extensions towards fermentation processes with (i) multiple substrates and (ii) non-monotonic kinetics are also included.
However, the cost of conducting piglet experiments can limit the use of this model to investigating multiple substrates.
When an enzyme has multiple substrates, inhibitors can show different types of inhibition depending on which substrate is considered.
These results suggest that the phosphorylated PPPSPXS motif inhibits GSK3 kinase activity towards multiple substrates.
The same kinetic trends were observed in ligase-mediated capture in complex reaction mixtures with multiple substrates.
However, understanding the exact role of DUBs in "real" cancers is complicated by the fact that DUBs have multiple substrates.
SRC activity in human tumors may further coordinate the phosphorylation of multiple substrates to mediate downstream phenotypes.
Perhaps, multiple substrates of PP1cγ may exist in the signaling pathways that control αIIbβ3 activation downstream of thrombin signaling.
Our numerical results for two enzyme-catalyzed reaction schemes involving multiple substrates and inhibitors further support our general theory.
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