Disrupted-In-Schizophrenia-1 protein occurs in multiple subcellular compartments, which include the centrosome, microtubule fractions, postsynaptic densities, actin cytoskeletal fractions, the mitochondria, and the nucleus.
In this study, we used D2O to simultaneously assess both new protein and new DNA synthesis across multiple tissues and subcellular fractions, as well as between sexes (Neese et al., 2002; Busch et al., 2006; Robinson et al., 2011; Miller et al., 2012a, b; Drake et al., 2013) in the long-lived Snell dwarf mouse.
To establish the subcellular localization of CII after uptake, macrophages were subjected to subcellular fractionation by Percoll density gradient centrifugation, and subcellular fractions were analyzed for markers characteristic of different subcellular compartments.
In contrast EHD4 was detected predominantly in type II cells with lower expression in the LB fraction, suggesting that EHD4 is distributed among multiple subcellular compartments in the type II cells (Figure 5D).
Subcellular fractions were obtained using the Subcellular Protein Fractionation Kit or the NE-PER Nuclear and Cytoplasmic Extraction Kit (Pierce Thermo Scientific) according to the manufacturer's instructions.
Subcellular fractions were prepared by differential ultracentrifugation.
Some drugs act on multiple subcellular targets.
To isolate iISGs and mISGs, the first step, the enrichment of subcellular fractions, was performed.
Samples can be enriched for selected subcellular fractions or for specific type of proteins (based on post-translational modifications).
Investigations of reactive intermediate formation in subcellular fractions with the use of radiolabeled reagents are also discussed.
