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Web services on MAMMOTH-mult server [40] were used to compute multiple structure alignments and additional pairwise structural alignments were made with SoFiSt program [72].
We introduce an algorithm that uses the information gained from simultaneous consideration of an entire group of related proteins to create multiple structure alignments (MSTAs).
(B) Multiple structure alignments for bovine arrestin-1 (PDB ID: 1CF1, red ribbon), arrestin-2 (PDB ID: 1G4M, green ribbon), arrestin-3 (PDB ID: 3P2D, cyan ribbon), and arrestin-4 (PDB ID: 1SUJ, orange ribbon).
These two conditions are inadequate for multiple structure alignments.
We then constructed a figure by performing multiple structure alignments on the ten sites having the highest similarity scores.
Results from multiple structure alignments for the same domain pair are averaged over the domain pair.
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Structures in each group were aligned by the multiple structure alignment function of RSmatch with default scoring matrices.
The sequences are aligned by way of multiple structure alignment program.
Of these, we removed the superfamilies 'Lipocalins' and 'Trypsin-like serine proteases,' which were not structurally consistent enough to permit a multiple structure alignment for training HMMER or the SMURF variants, and which were broken into distinct superfamilies by (Daniels et al., 2012), with the result that 11 superfamilies containing barrels were selected.
Multiple Structure Alignment presents all the reduced three-state assignments for each individual residue aligned in parallel to the protein sequence.
Multiple structure alignment was performed using the MultProt (http://bioinfo3d.cs.tau.ac.il/MultiProt/) (Shatsky et al., 2004).
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